The method of claim 13, further comprising isolating the complex.ġ6. The method of claim 13, wherein the method further comprises irradiating the cell thereby cleaving the ruthenium-based photolinker compound and exposing the antisense oligonucleotide under conditions suitable for binding of the antisense oligonucleotide to a nucleic acid of the cell, thereby forming a complex.ġ5. A method of capturing nucleic acid in a cell, the method comprising administering to the cell the composition of claim 1.ġ4. The molecule of claim 11, wherein the wherein a first end of the first stem-forming oligonucleotide is directly or indirectly linked to a first photolabile ligand of the ruthenium-based photolinker and wherein a first end of the second stem-forming oligonucleotide is directly or indirectly linked to a second photolabile ligand of the ruthenium-based photolinker.ġ3. The molecule of claim 1, wherein the molecule is a hairpin caged molecule comprising a stem and a loop, wherein the molecule comprises a first stem-forming oligonucleotide and a second stem-forming oligonucleotide, wherein the first stem-forming oligonucleotide and a second stem-forming oligonucleotide hybridize to form the stem and wherein the loop comprises the antisense oligonucleotide.ġ2. The molecule of claim 1, wherein irradiation of ruthenium-based photolinker exposes the antisense oligonucleotide thereby allowing the antisense oligonucleotide to hybridize with a nucleic acid.ġ1. The molecule of claim 1, wherein the antisense oligonucleotide comprises a randomized nucleotide sequence.ġ0. The molecule of claim 7, wherein the one or more fluorophores is a FRET pair.ĩ. The molecule of claim 1, further comprising one or more fluorophores.Ĩ. The molecule of claim 5, wherein the second end of the antisense oligonucleotide is directly or indirectly linked to a second photolabile ligand of the ruthenium-based photolinker.ħ. The molecule of claim 1, wherein the molecule is a circular caged molecule wherein a first end of the antisense oligonucleotide is directly or indirectly linked to a first photolabile ligand of the ruthenium-based photolinker and a second end of the antisense oligonucleotide is directly or indirectly linked elsewhere on the compound.Ħ. The molecule of claim 1, wherein the antisense oligonucleotide is at least an 18mer 2′fluoro oligonucleotide having a nucleic acid sequence that is substantially complementary to the polyA tail of an mRNA.ĥ. The molecule of claim 1, further comprising a cell penetrating domain.Ĥ. The molecule of claim 1 further comprising a label for isolating nucleic acids.ģ. A caged molecule for capturing one or more nucleic acid molecules, comprising an antisense oligonucleotide linked to a ruthenium-based photolinker compound of formula (II): wherein in formula (II): L 1, L 2, L 3, and L 4 are each independently a ligand and X 1 and X 2 are each independently a photolabile ligand having a reactive moiety.Ģ.
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